Beyond Conventional SEC: Deeper Molecular Insights for Biotherapeutics with SEC-MALS

As biotherapeutics become increasingly complex, understanding molecular size alone may not provide the complete characterization picture. From proteins and monoclonal antibodies to peptides and other emerging modalities, researchers need greater insight into molecular weight, aggregation, oligomeric state and sample heterogeneity to support confident development and characterization.

Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS) extends conventional SEC by enabling direct, calibration-independent determination of absolute molecular weight and deeper characterization of molecular species.

Join this webinar to explore how SEC-MALS works, what additional insights it can provide for biotherapeutic characterization, and how an integrated Agilent workflow brings together LC, SEC columns, MALS detection and software for confident analysis. Through practical application examples, discover how SEC-MALS can help characterize proteins and other biotherapeutic molecules, investigate aggregates and oligomers, and move beyond separation toward deeper molecular understanding.

Key Learnings:

Understand when and why to use SEC-MALS to address biotherapeutic characterization questions beyond conventional SEC analysis.

Learn how to build an effective SEC-MALS workflow, from SEC column selection and separation through MALS detection, data analysis and interpretation.

See how SEC-MALS is applied to real biotherapeutic challenges, and translate the resulting molecular insights into more informed characterization decisions.